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STUDY OF THE EFFECTS OF COMPLEX SULPHATED GLYCOSAMINOGLYCAN ON THE CULTURE OF THE EPITHELIUM OF THE HUMAN CORNEA IN VITRO

Annotation

Purpose : to investigate in vitro protective effect of sulfated glycosaminoglycans (sGAG) on human corneal epithelial cells exposed to BAK in vitro. Methods : experiment was carried out on the culture of the epithelium of the human cornea. In about 48 hours of culture alternation of culture medium with adding sample solution was made. In 1st test group was exposed to BAK 0.01 % + sGAG 0.1 %, in 2nd test group -BAK 0.01 %+sGAG 0.5 %, in 3d test group - BAK 0.01 % + sGAG 1 %, in 1st control group - 0.01 % BAK. For the real-time monitoring of cell growth and death we used the xCelligence real-time cell analysis system and phase-contrast microscopic study. Results : after 1 hour of treatment the cell index (CI) values in 1st test group (BAK + 0.1 % sGAG) reached of 0.87 ± 0.01 CI, in 2nd test group (BAK + 0.5 % sGAG) 0.91 ± 0.06 CI, in 3d test group (BAK + 1 % sGAG) 1.35 ± 0.26 CI , in 1st control group (BAK) - 0.71 ± 0.15 CI. After 2 hour of treatment the cell index (CI) values in 1st test group (BAK + 0.1 % sGAG) reached of 0.12 ± 0.01 CI, in 2nd test group (BAK + 0.5 % sGAG) 0.15 ± 0.10 CI, in 3rd test group (BAK + 1 % sGAG) 0.29 ± 0.01 CI, in control group (BAK) - 0.10 ± 0.07 CI. Conclusions : in this study, we determined the protective effects of sGAG on the corneal epithelial cell line exposed to BAK in vitro.

Keywords

sulfated glycosaminoglycans; benzalkonium chloride; toxicity; corneal epithelium

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DOI

10.20310/1810-0198-2016-21-4-1672-1677

UDC

617.72

Pages

1672-1677

References

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Received

2016-04-27

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