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PECULIARITIES OF CLINICAL PICTURE, OF INNATE IMMUNITY STATUS ON THE EXAMPLE OF TOLL RECEPTOR EXPRESSION ON PERIPHERAL BLOOD CELLS IN PATIENTS WITH UVEITIS OF DIFFERENT GENESIS

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Implementation specificity innate immune system falls into a family of evolutionarily conserved receptors, known as Toll-like receptors (TLR), which play a crucial role in the early protection of the organism against pathogens. Purpose : to study and carry out comparative characteristics of different types of expression of Toll receptors on peripheral blood cells in the control and uveitis of various etiologies. Material and Methods : the study involved 15 patients (7 men and 8 women) aged 25-54 years, with a diagnosis of acute or exacerbation of chronic uveitis of various etiologies. The control group consisted of healthy persons aged 23-27 years - 12 people. The level of expression of Toll-like receptor was evaluated by flow-cytometric analysis of leukocytes stained according to standard protocols fluorescently-labeled antibody (HyCult Biotech, The Netherlands) to the surface (TLR2, TLR4, TLR6) or intracellular (TLR3, TLR9) receptors. Results : the results obtained were statistically significant reduction TLR2 expression on B lymphocytes and monocytes ( p = 0.02 and 0.014, respectively) than two-times; TLR4 and TLR6 - on monocytes ( p = 0.01 and 0.047, respectively) in patients with infectious-toxic uveitis. Patients with severe autoimmune uveitis (6 persons) was there polymorphisms of expression different TLR and tended to increase compared to the control. Conclusion : in infectious uveitis most toxic pathogenetic importance is significant inhibition of the expression of TLR2, TLR4 and TLR6 on peripheral blood monocytes, which is possible due to the excessive stimulation of Toll receptors by exogenous ligands prior to or at the onset of the disease, and the development of state hyposensitivities Toll receptors, as a defensive reaction the uvea from hyperinflammation. Further studies of character expression Toll-receptors in uveitis remission is necessary in order to assess the influence of inflammatory activity and drug therapy on the expression TLR.

Keywords

Toll-like receptors; uveitis; peripheral blood cells; flow-cytometry

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DOI

10.20310/1810-0198-2016-21-4-1482-1487

UDC

617.7

Pages

1482-1487

References

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Received

2016-04-28

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Scholarly articles

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