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CORNEAL SAMPLE PREPARATION FOR SCANNING ELECTRON MICROSCOPY AFTER THE FORMATION OF A CORNEAL FLAP USING THE FEMTOSECOND LASER

Annotation

Eyes methods of sample preparation for corneal scanning electron microscopy after the flap formation using femtosecond laser were studied in the experiment on human cadaver. Samples 1 and 2 groups were fixed in 4.0 % formaldehyde solution, 3 and 4 - in the Ito-Karnovsky reagent. The samples 2 and 4 were also dehydrated using alcohol battery and critical point drying. No artifacts were found in 1 and 3 groups of samples. In 2 group on the surface of corneal stroma was identified certain “crumbs-looking” material, in 4 group - were identified crystal-like structures with length up to 15 microns and 0.8 microns in wide.

Keywords

scanning electron microscopy; cornea; corneal flap; femtosecond laser; femtoLASIK

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DOI

10.20310/1810-0198-2016-21-4-1492-1499

UDC

611.841.2

Pages

1492-1499

References

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Received

2016-03-18

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Scholarly articles

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