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THE INFLUENCE OF METHOTREXAT ON THE BRANCH OF WILD TYPE AND HYBRID BRANCHES OF HETEROZYGOUS ACCORDING TO THE GENE VESTIGIAL DROSOPHILA MELANOGASTER, RECEIVED IN THE PROCESS OF CONTROLLED BREEDING

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Drosophila melanogaster is a convenient model object for studying of consequences of the long directed selection because it has a short life cycle and fast alternation of generations. Many factors of a stress of the chemical and physical nature are capable to cause activization of movement of mobile genetic elements by the purpose of this work was to establish change of transpozon’s activity at a number of the D. melanogaster strains at methotrexate influence, or when crossing with the mutant strain vestigial during the long directed selection. The injuries like “cutting” on a wing were received in 2 ways: grew up individuals of wild type strains on the medium, which containing a methotrexate or crossed to individuals of the mutant vestigial (a sign rudimentary wings). Using the morphometric analysis of a wing showed the linear distinctions which are shown in response to the directed selection. Thus, the assumption of change of activity of transpozons is made, existence and localization in which all chromosomes were confirmed by PCR and FISH method. Emergence of mutations in hybrids, heterozygotic vestigial, also testifies in favor of this assumption.

Keywords

vestigial; methotrexate; transposons; vestigial

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DOI

10.20310/1810-0198-2016-21-5-1867-1870

UDC

575.22, 575.167

Pages

1867-1870

References

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Received

2016-05-24

Section of issue

Scientific articles. biology

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